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Yan Chen

 

 

 

 

Postdoctoral Fellow China

Biochemistry

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Research

Postdoctoral Fellow: Moffitt Cancer Center Tampa, FL. 2014 - Present

Research Assistant: Department of Chemistry at Georgia State University

Teaching Assistant: General Chemistry Laboratories at Georgia State University

Trainer of Undergraduate for Independent Research: at Georgia State University

Education

Biochemistry, Department of Chemistry

Georgia State University, GA (PhD) 08/2008-11/2013

Biochemistry, Department of Chemistry

Georgia State University GA (MS) 08/2008-11/2013

Botany, School of Life Science

Lanzhou University, Lanzhou, China (MS) 09/2006-07/2008

Biotechnology, School of Life Science

Lanzhou University, Lanzhou, China (BS) 09/2002-07/2006

Publications

Huo L, Fielding AJ, Chen Y, Li T, Iwaki H, Hosler JP, Chen L, Hasegawa Y, Que Jr, L, and Liu A (2012) Evidence for a dual role of an active site histidine in α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase. Biochemistry, 51(29), 5811-5821. (Shared first author).

Chen Y, Naik SG, Krzystek J, Shin S, Nelson WH, Xue S, Yang JJ, Davidson VL, and Liu A (2012) The role of calcium in metalloenzyme: Effects of calcium removal on the axial ligation geometry and magnatic properties of the catalytic diheme center in MauG. Biochemistry, 51(8), 1586-1597.

Shin S, Feng M, Chen Y, Jensen LM, Tachikawa H, Wilmot CM, Liu A, and Davidson VL (2011) The tightly bound calcium of MauG is required for tryptophan tryptophylquinone cofactor biosynthesis. Biochemistry, 50(1), 144-150.

Huang Y, Zhou Y, Wong HC, Chen, Y. Chen Y, Wang S, Castiblanco A, Liu A, Yang JJ (2009) A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+, FEBS J., 276(19), 5589-5597.

Resume

Professional Skills

Understanding the function of a second sphere residue H228 in a zinc containing decarboxylase using Resonance Raman, EPR and crystallography strategies

Crystallization of a de novo non-heme iron dependent enzyme -- cysteamine dioxygenase

Characterization of the role of the calcium ion in a di-heme enzyme MauG using electron paramagnetic resonance (EPR), Mössbauer and proton nuclear magnetic resonance (NMR)

Mechanistic study of a non-heme iron dependent thiol dioxygenase cysteamine dioxygenase (ADO) with EPR, Mössbauer, ICP-OES and mass spectrometry (ESI-MS, LC-MS).

Protein crystallization, remote data collection and molecular replacement refinement

X-band electron paramagnetic resonance (EPR), fluorescence and UV-Vis

Site-directed mutagenesis: plasmid extraction, PCR and transformation into E. Coli competent cells

Enzyme assay and kinetic study with Clark oxygen electrode 

Handling oxygen sensitive proteins with glove box and schlenk line

Protein chromatography on ÄKTA purifier

Protein expression and optimization in E.Coli strains

SDS-PAGE analysis of recombinant proteins

Use ofÄKTA-FPLC and purifier program, WinEPR, Spin (EPR spectrum simulation software), Chemdraw, Origin, PyMol, CCP4i, Phenix and Wincoot

Awards

Four-year undergraduate biotechnology base scholarship from Lanzhou University, China

 

Presentations

03/2012 The 1st CDT conference (Poster presentation: The role of calcium in metalloenzyme: Effects on the axial ligation geometry and magnetic properties of the catalytic di-heme center in MauG), Honorable mention for poster award

04/2011 The Calcium club, Georgia State University (Invited oral presentation: An unusual role of calcium in a di-heme enzyme, MauG)

03/2011 The NMR workshop, Georgia State University (Invited oral presentation: The tightly-bound Ca2+ of MauG profoundly influences the axial ligation geometry and magnetic properties of the catalytic di-heme center)

Affiliations

Judge of the SSS/McNaire Research Confrence, 2013, United States

 

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