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Paula Cranfill





Research Associate IUSA

Cellular and Molecular Biology



Research Associate I, H. Lee Moffitt Cancer Center, Tampa, FL, June 2014 – Present
Optical Microscopist, National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, 2009 - 2013



Chu J, Haynes RD, Corbel SY, Li P, González-González E, Burg JS, Ataie NJ, Lam AJ, Cranfill PJ, et al: Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. Nat Methods, 2014. PMID: 24633408.

Shaner NC, Lambert GG, Chammas A, Ni Y, Cranfill PJ, et al: A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods, 2013. PMID: 23524392.

McEvoy AL, Hoi H, Bates M, Platonova E, Cranfill PJ, et al: mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities. PLoS ONE, 2012. PMID: 23240015.

Shemiakina II, Ermakova GV, Cranfill PJ, et al: A monomeric red fluorescent protein with low cytotoxicity.  Nat Commun.,2012. PMID: 23149748.

Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, et al:  Improving FRET dynamic range with bright green and red fluorescent proteins.  Nat Methods, 2012. PMID: 22961245.

Kusi-Appiah AE, Vafai N, Cranfill PJ, Davidson MW, Lenhert S:  Lipid multilayer microarrays for in vitro liposomal drug delivery and screening.  Biomaterials, 2012. PMID: 22391265.

Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV:  An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore.  PLoS ONE, 2011. PMID: 22174863.

Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, et al:  An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.  PLoS ONE, 2011. PMID: 21479270.

Kremers GJ, Gilbert SG, Cranfill PJ, Davidson MW, Piston DW:  Fluorescent Proteins at a Glance.  Journal of Cell Science, 2011. PMID: 21187342.


Professional Skills

Proficient in DNA subcloning, immunofluorescence, SDS-PAGE, Western blot, IC50 cell viability assay, cell culture, and protein expression and purification.
Experience with fluorescent protein characterization: excitation and emission, quantum yield, extinction coefficient, pKa, photostability, and fluorescence resonance energy transfer (FRET) by acceptor photobleaching.
Optical microscopy: Köhler, DIC, phase contrast, epifluorescence, laser-scanning confocal, total internal reflection fluorescence (TIRF), and super-resolution.